Effects of fasting on liver glycogen structure in rats with type 2 diabetes.
Carbohydr Polym
; 237: 116144, 2020 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-32241436
ABSTRACT
Liver glycogen, a highly branched glucose polymer, is important for blood sugar homeostasis. It comprises α particles which are made of linked ß particles; the molecular structure changes diurnally. In diabetic liver, the α particles are fragile, easily breaking apart into ß particles in chaotropic agents such as dimethyl sulfoxide. We here use size-exclusion chromatography to study how fasting changes liver-glycogen structure in vivo for mice in which type-2 diabetes had previously been induced. Diabetic glycogen degraded enzymatically more quickly in the fasted animals than did glycogen without fasting, with fewer α particles, which however were still fragile. The glycogen had fewer long chains and more shorter chains after fasting. This study gives an overview of the in vivo dynamic changes in α-particles under starvation conditions in both normal and diabetic livers.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Jejum
/
Diabetes Mellitus Experimental
/
Diabetes Mellitus Tipo 2
/
Glicogênio Hepático
Limite:
Animals
Idioma:
En
Revista:
Carbohydr Polym
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China