Direct electrochemiluminescent immunosensing for an early indication of coronary heart disease using dual biomarkers.
Anal Chim Acta
; 1110: 82-89, 2020 May 08.
Article
em En
| MEDLINE
| ID: mdl-32278403
ABSTRACT
In this paper, a promising technology for obtaining an early indication and making a clinical diagnosis of coronary heart disease (CHD) is successfully designed. Specifically, label-free biosensors based on Au-Co nanoparticles (Au-Co NPs) were utilized to detect low-density lipoprotein (LDL) and oxidized low-density lipoprotein (ox-LDL), which are both CHD biomarkers. Conductive Au-Co NPs provided a prominent sensing platform and played the role of an efficient electrochemiluminescent (ECL) signaling amplifier. Conductive Au-Co NPs were fabricated using a simple method of water phase synthesis and were characterized using transmission electron microscope (TEM) and scanning electron microscope (SEM) images. An antibody was immobilized onto Au-Co NP-decorated indium tin oxide coated glass. After the formation of an immune complex between the antigen and antibody, using luminol as a sensing probe, it was found that the ECL signal was inhibited. Under the optimized conditions, the immunosensor exhibited sensitive detection of LDL over a wide linear range from 0.420 to 100 pg mL-1 with a detection limit of 0.256 pg mL-1. Likewise, a linear regression of the concentration of ox-LDL was obtained over the range from 0.500 pg mL-1 to 60.0 pg mL-1, with a detection limit of 0.330 pg mL-1. This research provides a new method for making a clinical diagnosis and obtaining an early indication of CHD due to the high performance sensing of these two biomarkers.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
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Doença das Coronárias
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Técnicas Eletroquímicas
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Medições Luminescentes
Limite:
Humans
Idioma:
En
Revista:
Anal Chim Acta
Ano de publicação:
2020
Tipo de documento:
Article
País de publicação:
HOLANDA
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HOLLAND
/
NETHERLANDS
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NL
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PAISES BAJOS
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THE NETHERLANDS