Improved high-performance liquid chromatographic determination of thiamine and its phosphate esters in animal tissues.
J Chromatogr
; 450(3): 317-23, 1988 Oct 26.
Article
em En
| MEDLINE
| ID: mdl-3241017
ABSTRACT
An improved method for the determination of thiamine and its phosphate esters in animal tissues using reversed-phase high-performance liquid chromatography with precolumn derivatization is described. Thiamine and its phosphate esters were converted into fluorophores by alkaline cyanogen bromide, and the derivatives were applied to an ODS packed column. Then the effluent obtained by an acidic mobile phase was mixed with an alkaline methanol solution to increase the fluorescence intensity of the derivatives which was determined spectrofluorometrically. A complete, rapid and quantitative separation of thiamin and its phosphate esters was achieved and the use of the acidic buffer as a mobile phase improved the column stability. The fluorophores of thiochrome ester peaks on the chromatogram were sensitive to pretreatment with thiamine triphosphatase or acid phosphatase. The applicability of the method to the determination of the form of thiamin in various tissues of rat is demonstrated.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tiamina
Limite:
Animals
Idioma:
En
Revista:
J Chromatogr
Ano de publicação:
1988
Tipo de documento:
Article
País de afiliação:
Japão