Graphene oxide size-dependently altered lipid profiles in THP-1 macrophages.

ABSTRACT

Previous studies focused on biocompatibility of graphene oxide (GO) to macrophages, but the impact of GO on lipid profiles in macrophages was less investigated. Herein, we investigated the interactions between THP-1 macrophages and GO of different sizes (GO of size 500-5000 nm, denoted as GO-L; GO of size < 500 nm, denoted as GO-S). We found that after 24 h exposure, the internalization of GO appeared to be minimal, whereas up to 50 µg/mL of GO-L but not GO-S reduced lipid accumulation, accompanying with a significantly reduced release of soluble monocyte chemoattractant protein-1 (MCP-1) but not interleukin-6 (IL-6). Moreover, lipidomic data showed that GO-L decreased the levels of 17 lipid classes, whereas GO-S only decreased the levels of 5 lipid classes. For comparison, 50 µg/mL carbon black (CB) significantly increased lipid accumulation with considerable particle internalization. GO-reduced lipid accumulation was not related with increase of reactive oxygen species (ROS) or induction of autophagy, and modulation of autophagy by chemicals showed no significant effect to alter the effects of GO-L on lipid accumulation. However, exposure to GO reduced the mRNA and protein levels of key components in peroxisome proliferators-activated receptor (PPAR) signaling pathway, a pathway that is related with lipid droplet biogenesis, and the modulation of PPARγ by chemicals altered the effects of GO-L on lipid accumulation. In conclusion, our results suggested that GO size-dependently altered lipid profiles in THP-1 macrophages that might be related with PPAR signaling pathway.