Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs.
Nat Plants
; 6(6): 620-624, 2020 06.
Article
em En
| MEDLINE
| ID: mdl-32483329
ABSTRACT
An in planta gene editing approach was developed wherein Cas9 transgenic plants are infected with an RNA virus that expresses single guide RNAs (sgRNAs). The sgRNAs are augmented with sequences that promote cell-to-cell mobility. Mutant progeny are recovered in the next generation at frequencies ranging from 65 to 100%; up to 30% of progeny derived from plants infected with a virus expressing three sgRNAs have mutations in all three targeted loci.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus de RNA
/
Nicotiana
/
RNA Viral
/
Plantas Geneticamente Modificadas
/
RNA Guia de Cinetoplastídeos
/
Edição de Genes
Idioma:
En
Revista:
Nat Plants
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Estados Unidos