Uncovering the chemistry of C-C bond formation in C-nucleoside biosynthesis: crystal structure of a C-glycoside synthase/PRPP complex.

Gao, Sisi; Radadiya, Ashish; Li, Wenbo; Liu, Huanting; Zhu, Wen; de Crécy-Lagard, Valérie; Richards, Nigel G J; Naismith, James H

Gao, Sisi; Radadiya, Ashish; Li, Wenbo; Liu, Huanting; Zhu, Wen; de Crécy-Lagard, Valérie; Richards, Nigel G J; Naismith, James H.

Afiliação

Gao S; Research Complex at Harwell, Didcot, OX11 0FA, UK and BSRC, University of St Andrews, St Andrews, KY16 9ST, UK.
Radadiya A; School of Chemistry, Cardiff University, Cardiff, CF10 3AT, UK.
Li W; Division of Structural Biology, University of Oxford, Oxford, OX3 7BN, UK. naismith@strubi.ox.ac.uk.
Liu H; BSRC, University of St Andrews, St Andrews, KY16 9ST, UK.
Zhu W; Department of Chemistry and California Institute for Quantitative Biosciences, University of California, Berkeley, CA 94720, USA.
de Crécy-Lagard V; Department of Microbiology, University of Florida, Gainesville, FL 32611, USA.
Richards NGJ; School of Chemistry, Cardiff University, Cardiff, CF10 3AT, UK and Foundation for Applied Molecular Evolution, Alachua, FL 32615, USA.
Naismith JH; Division of Structural Biology, University of Oxford, Oxford, OX3 7BN, UK. naismith@strubi.ox.ac.uk and The Rosalind Franklin Institute, Didcot, OX11 0FA, UK.

Chem Commun (Camb) ; 56(55): 7617-7620, 2020 Jul 14.

Article em En | MEDLINE | ID: mdl-32515440

ABSTRACT

ABSTRACT

The enzyme ForT catalyzes C-C bond formation between 5'-phosphoribosyl-1'-pyrophosphate (PRPP) and 4-amino-1H-pyrazole-3,5-dicarboxylate to make a key intermediate in the biosynthesis of formycin A 5'-phosphate by Streptomyces kaniharaensis. We report the 2.5 Å resolution structure of the ForT/PRPP complex and locate active site residues critical for PRPP recognition and catalysis.

Assuntos

Carbono-Carbono Ligases/metabolismo; Fosforribosil Pirofosfato/metabolismo; Proteínas de Bactérias/química; Proteínas de Bactérias/metabolismo; Biocatálise; Carbono-Carbono Ligases/química; Domínio Catalítico; Cristalografia por Raios X; Modelos Químicos; Fosforribosil Pirofosfato/química; Ligação Proteica; Streptomyces/enzimologia

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fosforribosil Pirofosfato / Carbono-Carbono Ligases Idioma: En Revista: Chem Commun (Camb) Assunto da revista: QUIMICA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Reino Unido

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