CHO; CHO cells; CHO, Chinese hamster ovary; Cell line development; Cell sorting; Chinese Hamster Ovary Cells; CoI, clusters of interest; DE, directed evolved; Directed Evolution; ECM, extracellular matrix; ES, enrichment score; FACS; FACS, fluorescent-activated cell sorting; Fluorescent-activated cell sorting; GSEA, gene set analysis; Growth enhancement; Growth improvement; LDC, limiting dilution cloning; NES, negative enrichment score; PC, principal component; PCA, principal component analysis; POI, product of interest; RNA Sequencing; RNA-Seq; RNA-Seq, RNA sequencing; SCC, single cell cloning; Single Cell Cloning; Single Cell Subcloning; Subcloning; lfcSE, logfoldstandard error