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Somatic copy number profiling from hepatocellular carcinoma circulating tumor cells.
Court, Colin M; Hou, Shuang; Liu, Lian; Winograd, Paul; DiPardo, Benjamin J; Liu, Sean X; Chen, Pin-Jung; Zhu, Yazhen; Smalley, Matthew; Zhang, Ryan; Sadeghi, Saeed; Finn, Richard S; Kaldas, Fady M; Busuttil, Ronald W; Zhou, Xianghong J; Tseng, Hsian-Rong; Tomlinson, James S; Graeber, Thomas G; Agopian, Vatche G.
Afiliação
  • Court CM; Department of Surgery, University of California, Los Angeles, Los Angeles, CA USA.
  • Hou S; Department of Surgery, Veteran's Health Administration, Greater Los Angeles, Los Angeles, CA USA.
  • Liu L; Department of Molecular, Cellular, and Integrative Physiology, University of California, Los Angeles, Los Angeles, CA USA.
  • Winograd P; Department of Surgery, University of California, Los Angeles, Los Angeles, CA USA.
  • DiPardo BJ; PacGenomics, llc, Los Angeles, CA USA.
  • Liu SX; Department of Surgery, University of California, Los Angeles, Los Angeles, CA USA.
  • Chen PJ; Department of Surgery, Veteran's Health Administration, Greater Los Angeles, Los Angeles, CA USA.
  • Zhu Y; Department of Surgery, University of California, Los Angeles, Los Angeles, CA USA.
  • Smalley M; Department of Surgery, Veteran's Health Administration, Greater Los Angeles, Los Angeles, CA USA.
  • Zhang R; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA USA.
  • Sadeghi S; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA USA.
  • Finn RS; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA USA.
  • Kaldas FM; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA USA.
  • Busuttil RW; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA USA.
  • Zhou XJ; Department of Medicine, Division of Hematology/Oncology, University of California, Los Angeles, Los Angeles, CA USA.
  • Tseng HR; Department of Medicine, Division of Hematology/Oncology, University of California, Los Angeles, Los Angeles, CA USA.
  • Tomlinson JS; Department of Surgery, University of California, Los Angeles, Los Angeles, CA USA.
  • Graeber TG; Department of Surgery, University of California, Los Angeles, Los Angeles, CA USA.
  • Agopian VG; California NanoSystems Institute, University of California, Los Angeles, Los Angeles, CA USA.
NPJ Precis Oncol ; 4: 16, 2020.
Article em En | MEDLINE | ID: mdl-32637655
ABSTRACT
Somatic copy number alterations (SCNAs) are important genetic drivers of many cancers. We investigated the feasibility of obtaining SCNA profiles from circulating tumor cells (CTCs) as a molecular liquid biopsy for hepatocellular carcinoma (HCC). CTCs from ten HCC patients underwent SCNA profiling. The Cancer Genome Atlas (TCGA) SCNA data were used to develop a cancer origin classification model, which was then evaluated for classifying 44 CTCs from multiple cancer types. Sequencing of 18 CTC samples (median 4 CTCs/sample) from 10 HCC patients using a low-resolution whole-genome sequencing strategy (median 0.88 million reads/sample) revealed frequent SCNAs in previously reported HCC regions such as 8q amplifications and 17p deletions. SCNA profiling revealed that CTCs share a median of 80% concordance with the primary tumor. CTCs had SCNAs not seen in the primary tumor, some with prognostic implications. Using a SCNA profiling model, the tissue of origin was correctly identified for 32/44 (73%) CTCs from 12/16 (75%) patients with different cancer types.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: NPJ Precis Oncol Ano de publicação: 2020 Tipo de documento: Article
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: NPJ Precis Oncol Ano de publicação: 2020 Tipo de documento: Article