A putative new SARS-CoV protein, 3c, encoded in an ORF overlapping ORF3a.
J Gen Virol
; 101(10): 1085-1089, 2020 10.
Article
em En
| MEDLINE
| ID: mdl-32667280
ABSTRACT
Identification of the full complement of genes in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a crucial step towards gaining a fuller understanding of its molecular biology. However, short and/or overlapping genes can be difficult to detect using conventional computational approaches, whereas high-throughput experimental approaches - such as ribosome profiling - cannot distinguish translation of functional peptides from regulatory translation or translational noise. By studying regions showing enhanced conservation at synonymous sites in alignments of SARS-CoV-2 and related viruses (subgenus Sarbecovirus) and correlating the results with the conserved presence of an open reading frame (ORF) and a plausible translation mechanism, a putative new gene - ORF3c - was identified. ORF3c overlaps ORF3a in an alternative reading frame. A recently published ribosome profiling study confirmed that ORF3c is indeed translated during infection. ORF3c is conserved across the subgenus Sarbecovirus, and encodes a 40-41 amino acid predicted transmembrane protein.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Homologia de Genes
/
Fases de Leitura
/
Betacoronavirus
Limite:
Humans
Idioma:
En
Revista:
J Gen Virol
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Reino Unido