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Caffeic acid phenethyl ester, a coffee polyphenol, inhibits DNA methylation in vitro and in vivo.
Wang, Pan; Yamabe, Noriko; Hong, Can-Jian; Bai, Hyoung-Woo; Zhu, Bao Ting.
Afiliação
  • Wang P; Shenzhen Key Laboratory of Steroid Drug Discovery and Development, School of Life and Health Sciences, The Chinese University of Hong Kong, Shenzhen, Guangdong, 518172, China. Electronic address: wangpan@cuhk.edu.cn.
  • Yamabe N; College of Korean Medicine, Gachon University, Seongnam, 13120, Republic of Korea.
  • Hong CJ; Shenzhen Key Laboratory of Steroid Drug Discovery and Development, School of Life and Health Sciences, The Chinese University of Hong Kong, Shenzhen, Guangdong, 518172, China.
  • Bai HW; Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup-si, Jeollabuk-do, 580-185, Republic of Korea.
  • Zhu BT; Shenzhen Key Laboratory of Steroid Drug Discovery and Development, School of Life and Health Sciences, The Chinese University of Hong Kong, Shenzhen, Guangdong, 518172, China; Department of Pharmacology, Toxicology and Therapeutics, School of Medicine, University of Kansas Medical Center, Kansas Cit
Eur J Pharmacol ; 887: 173464, 2020 Nov 15.
Article em En | MEDLINE | ID: mdl-32781171
DNA methylation represents an important epigenetic regulation of the genome. Earlier studies have suggested that dietary phenolic compounds including those contained in coffee, tea and soy products may modulate the level of DNA methylation. In this study, we first characterize the effect of caffeic acid phenethyl ester (CAPE) and other dietary phenolic compounds on DNA methylation in vitro. The IC50 values of CAPE, daidzein, isorhamnetin and genistein are 7.6, 6.9, 6.2, and 4.3 µM, respectively, in an in-vitro enzymatic assay system. Computational analysis indicates that CAPE, daidzein, isorhamnetin and genistein can bind inside the DNA substrate-binding site in human DNMT1 with a favorable binding energy. In an animal study, we find that maternal CAPE treatment shifts the coat color distribution of the 21-day-old Avy/a offspring towards the yellow phenotype, indicating that CAPE inhibits the methylation of the agouti gene promoter sequence in vivo. The results from this study may shed light on the potential epigenetic effect in the offspring resulting from maternal intake of certain coffee phenolics during pregnancy.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Álcool Feniletílico / Ácidos Cafeicos / Café / Metilação de DNA / Epigênese Genética / Polifenóis / Simulação de Acoplamento Molecular Limite: Animals / Female / Humans / Male / Pregnancy Idioma: En Revista: Eur J Pharmacol Ano de publicação: 2020 Tipo de documento: Article País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Álcool Feniletílico / Ácidos Cafeicos / Café / Metilação de DNA / Epigênese Genética / Polifenóis / Simulação de Acoplamento Molecular Limite: Animals / Female / Humans / Male / Pregnancy Idioma: En Revista: Eur J Pharmacol Ano de publicação: 2020 Tipo de documento: Article País de publicação: Holanda