NHEJ inhibitor SCR7 and its different forms: Promising CRISPR tools for genome engineering.
Gene
; 763: 144997, 2020 Dec 30.
Article
em En
| MEDLINE
| ID: mdl-32783992
ABSTRACT
The CRISPR-Cas system currently stands as one of the best multifaceted tools for site-specific genome engineering in mammals. An important aspect of research in this field focusses on improving the specificity and efficacy of precise genome editing in multiple model systems. The cornerstone of this mini-review is one of the extensively investigated small molecule inhibitor, SCR7, which abrogates NHEJ, a Ligase IV-dependent DSB repair pathway, thus guiding integration of the foreign DNA fragment via the more precise homology directed repair during genome editing. One of our recent studies sheds light on properties of different forms of SCR7. Here, we give a succinct account on the use of SCR7 and its different forms in CRISPR-Cas system, highlighting their chemical properties and biological relevance as potent efficiency-enhancing CRISPR tools.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pirimidinas
/
Bases de Schiff
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Inibidores Enzimáticos
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Reparo de DNA por Recombinação
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Sistemas CRISPR-Cas
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Edição de Genes
Limite:
Animals
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Humans
Idioma:
En
Revista:
Gene
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Índia