Method for Removing Thawed Human Milk From a Plastic Storage Bag Impacts Fat Retention.
J Pediatr Gastroenterol Nutr
; 72(2): 306-310, 2021 02 01.
Article
em En
| MEDLINE
| ID: mdl-32826801
ABSTRACT
OBJECTIVES:
Determine how thaw stage and bag manipulation (folding and squeezing) influence the retention of fat and number of aerobic bacteria colony-forming units when decanting human milk (HM) from plastic storage bags.METHODS:
Lactating women (nâ=â40) in the Greensboro, North Carolina area were recruited to provide fresh HM. Samples were equally divided and frozen in storage bags for 2 months. Two thaw stages (ice/liquid) and the use of bag manipulation (yes/no) were assessed. Fat was measured using ether extraction and bacteria were measured using plate enumeration. Paired t tests were used to compare the effects of thaw stage and bag manipulation on post-thaw fat content. Repeated measures analysis of variance was used to compare the effect of bag manipulation on pre- and post-thaw bacteria.RESULTS:
Fat retention was not significantly different when thawing to liquid versus ice (mean differenceâ=â0.10âg/dL; nâ=â17 paired samples; Pâ=â0.07). Decanting with bag manipulation retained more fat than decanting without manipulation, but only when HM was thawed to liquid (mean differenceâ=â0.13âg/dL; nâ=â11 paired samples; Pâ=â0.005), not when HM was thawed to ice (Pâ=â0.47). Bag manipulation did not increase total aerobic bacteria for either thaw stage (Pâ=â0.49).CONCLUSIONS:
Fat retention is influenced by the method of removing previously frozen HM from plastic storage bags. Folding and squeezing the storage bag when decanting HM thawed to a liquid state increases fat recovery without increasing bacterial contamination.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Lactação
/
Leite Humano
Limite:
Female
/
Humans
País/Região como assunto:
America do norte
Idioma:
En
Revista:
J Pediatr Gastroenterol Nutr
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Nova Caledônia