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DEPS-1 is required for piRNA-dependent silencing and PIWI condensate organisation in Caenorhabditis elegans.
Suen, Kin Man; Braukmann, Fabian; Butler, Richard; Bensaddek, Dalila; Akay, Alper; Lin, Chi-Chuan; Milonaityte, Dovile; Doshi, Neel; Sapetschnig, Alexandra; Lamond, Angus; Ladbury, John Edward; Miska, Eric Alexander.
Afiliação
  • Suen KM; Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QN, UK.
  • Braukmann F; Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK.
  • Butler R; Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QN, UK.
  • Bensaddek D; Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK.
  • Akay A; Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QN, UK.
  • Lin CC; Laboratory for Quantitative Proteomics, Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, UK.
  • Milonaityte D; Bioscience Core labs, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia.
  • Doshi N; Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QN, UK.
  • Sapetschnig A; Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK.
  • Lamond A; School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK.
  • Ladbury JE; School of Molecular and Cellular Biology, University of Leeds, LC Miall Building, Leeds, LS2 9JT, UK.
  • Miska EA; School of Molecular and Cellular Biology, University of Leeds, LC Miall Building, Leeds, LS2 9JT, UK.
Nat Commun ; 11(1): 4242, 2020 08 25.
Article em En | MEDLINE | ID: mdl-32843637
ABSTRACT
Membraneless organelles are sites for RNA biology including small non-coding RNA (ncRNA) mediated gene silencing. How small ncRNAs utilise phase separated environments for their function is unclear. We investigated how the PIWI-interacting RNA (piRNA) pathway engages with the membraneless organelle P granule in Caenorhabditis elegans. Proteomic analysis of the PIWI protein PRG-1 reveals an interaction with the constitutive P granule protein DEPS-1. DEPS-1 is not required for piRNA biogenesis but piRNA-dependent silencing deps-1 mutants fail to produce the secondary endo-siRNAs required for the silencing of piRNA targets. We identify a motif on DEPS-1 which mediates a direct interaction with PRG-1. DEPS-1 and PRG-1 form intertwining clusters to build elongated condensates in vivo which are dependent on the Piwi-interacting motif of DEPS-1. Additionally, we identify EDG-1 as an interactor of DEPS-1 and PRG-1. Our study reveals how specific protein-protein interactions drive the spatial organisation and piRNA-dependent silencing within membraneless organelles.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Caenorhabditis elegans / Inativação Gênica / Proteínas de Caenorhabditis elegans / RNA Interferente Pequeno / Proteínas Argonautas Limite: Animals Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Reino Unido
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Caenorhabditis elegans / Inativação Gênica / Proteínas de Caenorhabditis elegans / RNA Interferente Pequeno / Proteínas Argonautas Limite: Animals Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Reino Unido