Your browser doesn't support javascript.
loading
Atlantic salmon kidney (ASK) cells are an effective model to characterise interferon (IFN) and IFN-induced gene expression following salmonid alphavirus infection.
Munir, Danish; Munro, Eann S; Secombes, Christopher J; Dooley, Helen.
Afiliação
  • Munir D; School of Biological Sciences, University of Aberdeen, Aberdeen, UK; Scottish Fish Immunology Research Centre, University of Aberdeen, Aberdeen, UK.
  • Munro ES; Marine Scotland Science, Marine Laboratory, Aberdeen, UK.
  • Secombes CJ; School of Biological Sciences, University of Aberdeen, Aberdeen, UK; Scottish Fish Immunology Research Centre, University of Aberdeen, Aberdeen, UK.
  • Dooley H; Dept Microbiology & Immunology, University of Maryland School of Medicine, Institute of Marine & Environmental Technology, Baltimore, USA. Electronic address: HDooley@som.umaryland.edu.
Fish Shellfish Immunol ; 106: 792-795, 2020 Nov.
Article em En | MEDLINE | ID: mdl-32871248
Salmonid alphavirus (SAV), the causative agent of pancreas disease, is a serious pathogen of farmed Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss). Given the economic impact of SAV outbreaks, much effort is focussed upon understanding the fish immune response following infection and the exploitation of this knowledge to reduce disease impact. Herein we examine the utility of the long-term Atlantic salmon kidney (ASK) cell line as a tool to study antiviral responses upon infection with SAV. Following infection with SAV subtype 1 (isolate V4640) we examined the kinetics and magnitude of induction of IFNa, IFN-regulatory factor (IRF) genes IRF1, IRF3, and IRF7b, as well as the antiviral effector Mx by RT-qPCR. SAV-1 non-structural protein (nsp1) transcript levels increased continuously over the experimental period, indicating viral replication, but cytopathic effect (CPE) was not observed. All the immune genes studied showed an increase in transcript levels over the 96-h study period following SAV infection, with strongest induction of Mx. Our data confirm that ASK cells are a suitable model to study the virus-associated immune responses of salmonids and may be a useful tool when assaying the effectiveness of potential prophylactic or antiviral treatments.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Interferons / Infecções por Alphavirus / Salmo salar / Doenças dos Peixes / Rim Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Fish Shellfish Immunol Assunto da revista: BIOLOGIA / MEDICINA VETERINARIA Ano de publicação: 2020 Tipo de documento: Article País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Interferons / Infecções por Alphavirus / Salmo salar / Doenças dos Peixes / Rim Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Fish Shellfish Immunol Assunto da revista: BIOLOGIA / MEDICINA VETERINARIA Ano de publicação: 2020 Tipo de documento: Article País de publicação: Reino Unido