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Development of a qualitative real-time RT-PCR assay for the detection of SARS-CoV-2: A guide and case study in setting up an emergency-use, laboratory-developed molecular assay.
Anahtar, Melis N; Shaw, Bennett; Slater, Damien; Byrne, Elizabeth; Botti-Lodovico, Yolanda; Adams, Gordon; Schaffner, Stephen; Eversley, Jacqueline; McGrath, Graham; Gogakos, Tasos; Lennerz, Jochen; Desai Marble, Hetal; Ritterhouse, Lauren L; Batten, Julie; Georgantas, N Zeke; Pellerin, Rebecca; Signorelli, Sylvia; Thierauf, Julia; Kemball, Molly; Happi, Christian; Grant, Donald S; Ndiaye, Daouda; Siddle, Katherine J; Mehta, Samar B; Harris, Jason; Ryan, Edward T; Pierce, Virginia; LaRocque, Regina; Lemieux, Jacob E; Sabeti, Pardis; Rosenberg, Eric; Branda, John; Turbett, Sarah E.
Afiliação
  • Anahtar MN; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts.
  • Shaw B; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.
  • Slater D; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA.
  • Byrne E; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA.
  • Botti-Lodovico Y; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.
  • Adams G; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.
  • Schaffner S; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.
  • Eversley J; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA.
  • McGrath G; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.
  • Gogakos T; Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA.
  • Lennerz J; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Harvard University, Boston, Massachusetts, USA.
  • Desai Marble H; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts.
  • Ritterhouse LL; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA.
  • Batten J; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts.
  • Georgantas NZ; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts.
  • Pellerin R; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts.
  • Signorelli S; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts.
  • Thierauf J; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts.
  • Kemball M; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts.
  • Happi C; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts.
  • Grant DS; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts.
  • Ndiaye D; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts.
  • Siddle KJ; Department of Otorhinolaryngology, Head and Neck Surgery, Experimental Head and Neck Oncology, Heidelberg University Hospital, Heidelberg, Germany.
  • Mehta SB; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.
  • Harris J; Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA.
  • Ryan ET; Department of Biological Sciences, Redeemer's University, Ede, Osun State, Nigeria.
  • Pierce V; African Center of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.
  • LaRocque R; Viral Hemorrhagic Fever Program, Kenema Government Hospital, Ministry of Health and Sanitation, 1 Combema Road, Kenema, Sierra Leone.
  • Lemieux JE; College of Medicine and Allied Health Sciences, University of Sierra Leone, Freetown, Sierra Leone.
  • Sabeti P; African Center of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.
  • Rosenberg E; Université Cheikh Anta Diop, BP 5005, Dakar, Sénégal.
  • Branda J; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.
  • Turbett SE; Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA.
medRxiv ; 2020 Sep 01.
Article em En | MEDLINE | ID: mdl-32909014

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Qualitative_research Idioma: En Revista: MedRxiv Ano de publicação: 2020 Tipo de documento: Article País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Qualitative_research Idioma: En Revista: MedRxiv Ano de publicação: 2020 Tipo de documento: Article País de publicação: Estados Unidos