Regenerative Potential of a Suspension and Spheroids of Multipotent Mesenchymal Stromal Cells from Human Umbilical Cord on the Model of Myocardial Infarction in Rats.

Krasina, M E; Kosheleva, N V; Lipina, T V; Karganov, M Yu; Medvedeva, Yu S; Lebedeva, M A; Zurina, I M; Saburina, I N

Krasina, M E; Kosheleva, N V; Lipina, T V; Karganov, M Yu; Medvedeva, Yu S; Lebedeva, M A; Zurina, I M; Saburina, I N.

Afiliação

Krasina ME; Faculty of Biology, M. V. Lomonosov Moscow State University, Moscow, Russia. mkrasina@icloud.com.
Kosheleva NV; Faculty of Biology, M. V. Lomonosov Moscow State University, Moscow, Russia.
Lipina TV; Research Institute of General Pathology and Pathophysiology, Moscow, Russia.
Karganov MY; Russian Medical Academy of Continuous Professional Education, Ministry of Health of the Russian Federation, Moscow, Russia.
Medvedeva YS; Faculty of Biology, M. V. Lomonosov Moscow State University, Moscow, Russia.
Lebedeva MA; Research Institute of General Pathology and Pathophysiology, Moscow, Russia.
Zurina IM; Research Institute of General Pathology and Pathophysiology, Moscow, Russia.
Saburina IN; Research Institute of General Pathology and Pathophysiology, Moscow, Russia.

Bull Exp Biol Med ; 169(4): 549-557, 2020 Aug.

Article em En | MEDLINE | ID: mdl-32910392

ABSTRACT

ABSTRACT

Regenerative potential of multipotent mesenchymal stromal cells from the human umbilical cord (MMSC-UC) in the suspension and spheroid form was revealed during the progression of experimental small focal myocardial infarction in rats. In isoproterenol-induced myocardial infarction, foci of necrosis and inflammatory infiltrate and at later terms fibrosis foci were found mainly in the left ventricle of rat heart. In rats receiving MMSC-UC, destructive changes in the myocardium, fibrous scars, and inflammatory process were less pronounced. MMSC-UC also contributed to normalization of the morphofunctional parameters of the heart. Spheroids exhibited higher efficiency in comparison with cell suspension.

Assuntos

Fibrose Endomiocárdica/prevenção & controle; Transplante de Células-Tronco Mesenquimais; Células-Tronco Mesenquimais/fisiologia; Infarto do Miocárdio/terapia; Regeneração/fisiologia; Esferoides Celulares/transplante; Animais; Modelos Animais de Doenças; Fibrose Endomiocárdica/induzido quimicamente; Fibrose Endomiocárdica/patologia; Fibrose Endomiocárdica/fisiopatologia; Ventrículos do Coração/patologia; Ventrículos do Coração/ultraestrutura; Humanos; Isoproterenol/administração & dosagem; Masculino; Células-Tronco Mesenquimais/citologia; Infarto do Miocárdio/induzido quimicamente; Infarto do Miocárdio/patologia; Infarto do Miocárdio/fisiopatologia; Miocárdio/patologia; Miocárdio/ultraestrutura; Miócitos Cardíacos/patologia; Miócitos Cardíacos/ultraestrutura; Cultura Primária de Células; Ratos; Ratos Wistar; Esferoides Celulares/citologia; Esferoides Celulares/fisiologia; Transplante Heterólogo; Resultado do Tratamento; Cordão Umbilical/citologia; Cordão Umbilical/metabolismo

Palavras-chave

isoproterenol; multipotent mesenchymal stromal cells; myocardial infarction; spheroids

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regeneração / Esferoides Celulares / Transplante de Células-Tronco Mesenquimais / Fibrose Endomiocárdica / Células-Tronco Mesenquimais / Infarto do Miocárdio Limite: Animals / Humans / Male Idioma: En Revista: Bull Exp Biol Med Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Federação Russa

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