FSH1 encodes lysophospholipase activity in Saccharomyces cerevisiae.
Biotechnol Lett
; 43(1): 279-286, 2021 Jan.
Article
em En
| MEDLINE
| ID: mdl-32920715
ABSTRACT
OBJECTIVES:
To elucidate the role of FSH1 (family of serine hydrolase) in lipid homeostasis.RESULTS:
Proteins in various species containing alpha/beta hydrolase domain are known to be involved in lipid metabolism. In silico analysis of the FSH1 gene in Saccharomyces cerevisiae revealed the presence of alpha/beta hydrolase domain (ABHD) and a lipase motif (GXSXG), however its function in lipid metabolism remained elusive. The overexpression of FSH1 in WT and fsh1Δ cells showed a significant reduction in the cellular phospholipid levels and an increase in the triacylglycerol levels and lipid droplet (LD) number. Furthermore, the purified recombinant protein Fsh1p was identified as a lysophospholipase that specifically acts on lysophosphatidylserine (LPS) and impacts the lipid homeostasis in S. cerevisiae.CONCLUSIONS:
These results depicted that Fsh1p has a role on lipid homeostasis and is a lysophospholipase that hydrolyzes lysophosphatidylserine (LPS).Palavras-chave
Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Proteínas de Saccharomyces cerevisiae
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Serina Proteases
/
Lisofosfolipase
Idioma:
En
Revista:
Biotechnol Lett
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Índia