[Determination of 4 polycyclic aromatic hydrocarbons in spicy strips by vacuum concentration coupled with isotope dilution gas chromatography-mass spectrometry].

ABSTRACT

OBJECTIVE: A method based on isotope internal standard dilution was established for the determination of four polycyclic aromatic hydrocarbons(PAH4) including chrysene, benzo [a] anthracene, benzo [b] fluoranthene and benzo [a] pyrene in spicy strips sold in the markets. METHODS: The hot strips were homogenized and the target compounds were extracted with n-hexane, concentrated in vacuum, saponified and purified by solid phase extraction column, then the pretreated samples were separated by DB-EUPAH column(20 m×0. 18 mm, 0. 14 µm), detected by gas chromatography-mass spectrometry(GC-MS)and quantified by internal standard of isotope. RESULTS: The recoveries of PAH4 in different concentration levels of hot noodles were 91. 0%-103. 5%, and the relative standard deviation(RSD)was 1. 89%-6. 73%(n=6). The detection limit was 0. 30 µg/kg and the quantitative limit was 1. 0 µg/kg. The content of PAH4(sum) in 27 collected samples ranged from 1. 35 µg/kg to 11. 44 µg/kg, and the detection rate was 100%. CONCLUSION: With less solvent consumption, good purification effect and blank control, the method is simple, rapid and accurate and meets the detecting requirements of PAH4 in hot strips.