Establishment of a PEG-mediated protoplast transformation system based on DNA and CRISPR/Cas9 ribonucleoprotein complexes for banana.

Wu, Shaoping; Zhu, Haocheng; Liu, Jinxing; Yang, Qiaosong; Shao, Xiuhong; Bi, Fangcheng; Hu, Chunhua; Huo, Heqiang; Chen, Kunling; Yi, Ganjun

Wu, Shaoping; Zhu, Haocheng; Liu, Jinxing; Yang, Qiaosong; Shao, Xiuhong; Bi, Fangcheng; Hu, Chunhua; Huo, Heqiang; Chen, Kunling; Yi, Ganjun.

Afiliação

Wu S; College of Horticulture, Hunan Agricultural University, Changsha, China.
Zhu H; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization (Ministry of Agriculture and Rural Affairs), Guangdong Key Laboratory of Tropical and Subtropical Fruit Tree Research, Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Liu J; State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
Yang Q; University of Chinese Academy of Sciences, Beijing, China.
Shao X; State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
Bi F; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization (Ministry of Agriculture and Rural Affairs), Guangdong Key Laboratory of Tropical and Subtropical Fruit Tree Research, Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Hu C; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization (Ministry of Agriculture and Rural Affairs), Guangdong Key Laboratory of Tropical and Subtropical Fruit Tree Research, Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Huo H; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization (Ministry of Agriculture and Rural Affairs), Guangdong Key Laboratory of Tropical and Subtropical Fruit Tree Research, Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Chen K; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization (Ministry of Agriculture and Rural Affairs), Guangdong Key Laboratory of Tropical and Subtropical Fruit Tree Research, Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Yi G; Mid-Florida Research and Education Center, University of Florida, Apopka, FL, USA.

BMC Plant Biol ; 20(1): 425, 2020 Sep 15.

Article em En | MEDLINE | ID: mdl-32933485

Assuntos

Proteína 9 Associada à CRISPR/genética; Proteína 9 Associada à CRISPR/metabolismo; Musa/genética; Musa/metabolismo; Polietilenoglicóis/metabolismo; Protoplastos/metabolismo; Ribonucleoproteínas/genética; Ribonucleoproteínas/metabolismo; Produtos Agrícolas/genética; Produtos Agrícolas/metabolismo; Regulação da Expressão Gênica de Plantas; Mutagênese Sítio-Dirigida/métodos; Melhoramento Vegetal/métodos

Palavras-chave

DNA-free; Deep amplicon sequencing; Genome editing; PEG-mediated; Protoplast transformation

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polietilenoglicóis / Protoplastos / Ribonucleoproteínas / Musa / Proteína 9 Associada à CRISPR Idioma: En Revista: BMC Plant Biol Assunto da revista: BOTANICA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: China País de publicação: Reino Unido

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