Reduction of Taylor-Aris dispersion by lateral mixing for chromatographic applications.
Lab Chip
; 20(21): 3938-3947, 2020 11 07.
Article
em En
| MEDLINE
| ID: mdl-32975255
ABSTRACT
Chromatographic columns are suffering from Taylor-Aris dispersion, especially for slowly diffusing molecules such as proteins. Since downscaling the channel size to reduce Taylor-Aris dispersion meets fundamental pressure limitations, new strategies are needed to further improve chromatography beyond its current limits. In this work we demonstrate a method to reduce Taylor-Aris dispersion by lateral mixing in a newly designed silicon AC-electroosmotic flow mixer. We obtained a reduction in κaris by a factor of three in a 40 µm × 20 µm microchannel, corresponding to a plate height gain of 2 to 3 under unretained conditions at low to high Pe values. We also demonstrate an improvement of a reverse-phase chromatographic separation of coumarins.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Lab Chip
Assunto da revista:
BIOTECNOLOGIA
/
QUIMICA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Bélgica