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Accurate and sensitive detection of Salmonella in foods by engineered bacteriophages.
Nguyen, Minh M; Gil, Jose; Brown, Matthew; Cesar Tondo, Eduardo; Soraya Martins de Aquino, Nathanyelle; Eisenberg, Marcia; Erickson, Stephen.
Afiliação
  • Nguyen MM; Laboratory Corporation of America Holdings, New Brighton, MN, 55112, USA.
  • Gil J; Laboratory Corporation of America Holdings, Los Angeles, CA, 90062, USA.
  • Brown M; Laboratory Corporation of America Holdings, Burlington, NC, 27215, USA.
  • Cesar Tondo E; Laboratório de Microbiologia e Controle de Alimentos, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul (ICTA/UFRGS), Porto Alegre, RS, 91501-970, Brazil.
  • Soraya Martins de Aquino N; Laboratório de Microbiologia e Controle de Alimentos, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul (ICTA/UFRGS), Porto Alegre, RS, 91501-970, Brazil.
  • Eisenberg M; Laboratory Corporation of America Holdings, Burlington, NC, 27215, USA.
  • Erickson S; Laboratory Corporation of America Holdings, New Brighton, MN, 55112, USA. erickss@labcorp.com.
Sci Rep ; 10(1): 17463, 2020 10 15.
Article em En | MEDLINE | ID: mdl-33060781
Salmonella is a major causative agent of foodborne illness and rapid identification of this pathogen is essential to prevent disease. Currently most assays require high bacterial burdens or prolonged enrichment to achieve acceptable performance. A reduction in testing time without loss of sensitivity is critical to allow food processors to safely decrease product holding time. To meet this need, a method was developed to detect Salmonella using luciferase reporter bacteriophages. Bacteriophages were engineered to express NanoLuc, a novel optimized luciferase originating from the deep-sea shrimp Oplophorus gracilirostris. NanoLuc-expressing bacteriophages had a limit of detection of 10-100 CFU per mL in culture without enrichment. Luciferase reporters demonstrated a broad host range covering all Salmonella species with one reporter detecting 99.3% of 269 inclusivity strains. Cross-reactivity was limited and only observed with other members of the Enterobacteriaceae family. In food matrix studies, a cocktail of engineered bacteriophages accurately detected 1 CFU in either 25 g of ground turkey with a 7 h enrichment or 100 g of powdered infant formula with a 16 h enrichment. Use of the NanoLuc reporter assay described herein resulted in a considerable reduction in enrichment time without a loss of sensitivity.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Produtos Avícolas / Salmonella / Bacteriófagos / Contaminação de Alimentos / Alimentos Marinhos / Fórmulas Infantis / Microbiologia de Alimentos Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Produtos Avícolas / Salmonella / Bacteriófagos / Contaminação de Alimentos / Alimentos Marinhos / Fórmulas Infantis / Microbiologia de Alimentos Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido