Development of an optimized closed and semi-automatic protocol for Good Manufacturing Practice manufacturing of tumor-infiltrating lymphocytes in a hospital environment.
Cytotherapy
; 22(12): 780-791, 2020 12.
Article
em En
| MEDLINE
| ID: mdl-33069566
ABSTRACT
BACKGROUND AIMS:
Several studies report on Good Manufacturing Process (GMP)-compliant manufacturing protocols for the ex vivo expansion of tumor-infiltrating lymphocytes (TILs) for the treatment of patients with refractory melanoma and other solid malignancies. Further opportunities for improvements in terms of ergonomy and operating time have been identified.METHODS:
To enable GMP-compliant TILs production for adoptive cell therapy needs, a simple automated and reproducible protocol for TILs manufacturing with the use of a closed system was developed and implemented at the authors' institution.RESULTS:
This protocol enabled significant operating time reduction during TILs expansion while allowing the generation of high-quality TILs products.CONCLUSIONS:
A simplified and efficient method of TILs expansion will enable the broadening of individualized tumor therapy and will increase patients' access to state-of-the-art TILs adoptive cell therapy treatment.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos do Interstício Tumoral
/
Técnicas de Cultura de Células
/
Hospitais
Tipo de estudo:
Guideline
Limite:
Female
/
Humans
Idioma:
En
Revista:
Cytotherapy
Assunto da revista:
TERAPEUTICA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Suíça