A new water-soluble bacterial NADH: fumarate oxidoreductase.
FEMS Microbiol Lett
; 367(20)2020 11 11.
Article
em En
| MEDLINE
| ID: mdl-33107907
ABSTRACT
The cytoplasmic fumarate reductase of Klebsiella pneumoniae (FRD) is a monomeric protein which contains three prosthetic groups noncovalently bound FMN and FAD plus a covalently bound FMN. In the present work, NADH is revealed to be an inherent electron donor for this enzyme. We found that the fumarate reductase activity of FRD significantly exceeds its NADH dehydrogenase activity. During the catalysis of NADHfumarate oxidoreductase reaction, FRD turnover is limited by a very low rate (â¼10/s) of electron transfer between the noncovalently and covalently bound FMN moieties. Induction of FRD synthesis in K. pneumoniae cells was observed only under anaerobic conditions in the presence of fumarate or malate. Enzymes with the FRD-like domain architecture are widely distributed among various bacteria and apparently comprise a new type of water-soluble NADHfumarate oxidoreductases.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Klebsiella pneumoniae
/
NAD
Idioma:
En
Revista:
FEMS Microbiol Lett
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Federação Russa