Convenient Protocol for Production and Purification of Clostridioides difficile Spores for Germination Studies.
STAR Protoc
; 1(2): 100071, 2020 09 18.
Article
em En
| MEDLINE
| ID: mdl-33111107
ABSTRACT
Clostridioides difficile, an obligate anaerobic bacterium, causes infections leading to prolonged diarrhea. The bacterium produces dormant spores that can withstand an aerobic environment, resulting in easy environmental transfer. Here, we present a convenient sporulation and purification protocol that can be practiced in any lab setting using a portable anaerobic glove bag. This protocol also optimizes existing cell growth methods and presents a detailed trouble shooting guide. This protocol is a modification of those previously reported by Edwards and McBride (2016) and Shen et al. (2016).
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Esporos Bacterianos
/
Clostridioides difficile
/
Técnicas Bacteriológicas
/
Técnicas de Cultura de Células
Tipo de estudo:
Guideline
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Estados Unidos