Gain-of-function assay for SARS-CoV-2 M <sup>pro</sup> inhibition in living cells.

Moghadasi, Seyad Arad; Becker, Jordan T; Belica, Christopher; Wick, Chloe; Brown, William L; Harris, Reuben S

Gain-of-function assay for SARS-CoV-2 M ^pro inhibition in living cells.

Moghadasi, Seyad Arad; Becker, Jordan T; Belica, Christopher; Wick, Chloe; Brown, William L; Harris, Reuben S.

Afiliação

Moghadasi SA; Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA, 55455.
Becker JT; Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA, 55455.
Belica C; Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA, 55455.
Wick C; Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA, 55455.
Brown WL; Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA, 55455.
Harris RS; Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA, 55455.

bioRxiv ; 2020 Nov 09.

Article em En | MEDLINE | ID: mdl-33200129

ABSTRACT

ABSTRACT

The main protease, M pro , of SARS-CoV-2 is required to cleave the viral polyprotein into precise functional units for virus replication and pathogenesis. Here we demonstrate a quantitative reporter for M pro function in living cells, in which protease inhibition by genetic or chemical methods results in strong eGFP fluorescence. This robust gain-of-function system readily distinguishes between inhibitor potencies and can be scaled-up to high-throughput platforms for drug testing.

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: BioRxiv Ano de publicação: 2020 Tipo de documento: Article