[Synergistic Antibacterial Activity of Berberine in Combination with Amylmetacresol Against Enterococcus faecalis in vitro].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 51(6): 749-754, 2020 Nov.
Article
em Zh
| MEDLINE
| ID: mdl-33236595
ABSTRACT
OBJECTIVE:
To study the antibacterial effect of berberine combined with amylmetacresol on Enterococcus faecalis.METHODS:
Both dilution method and live bacteria CFU were used to determine the minimum inhibitory concentration (MIC) of berberine and amylmetacresol on E. faecalis. The killing effect of berberine and amylmetacresol on planktonic E. faecalis was detected by suspension quantitative germicidal test and live/dead bacteria staining. The effects of berberine and amylmetacresol on the structure of mature biofilm of E. faecalis was observed by scanning electron microscopy (SEM). The toxicity of berberine and amylmetacresol on human oral keratinocytes (HOK) was determined by CCK-8 cell proliferation and cytotoxicity assay and cytotoxicity LDH assay.RESULTS:
The MIC of berberine was 512 µg/mL, and the MIC of amylmetacresol was 0.023 3%. 512 µg/mL berberine and 0.002 33% amylmetacresol had a weak killing effect on planktonic E. faecalis alone, while they showed a synergistic antibacterial effect in combination. Cell survival in the biofilm was only slightly changed by berberine and amylmetacresol. The structure of biofilm was obviously changed by berberine and amylmetacresol. 512 µg/mL berberine and 0.002 33% amylmetacresol alone or in combination showed the survival rate was much higher than the injury rate, suggesting berberine and amylmetacresol had a low cytotoxicity.CONCLUSION:
Berberine and amylmetacresol had synergism against E. faecalis, and the biological safety of the combination use was better.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Berberina
/
Enterococcus faecalis
Limite:
Humans
Idioma:
Zh
Revista:
Sichuan Da Xue Xue Bao Yi Xue Ban
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China