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Enhanced neural differentiation of neural stem cells by sustained release of Shh from TG2 gene-modified EMSC co-culture in vitro.
Shi, Wentao; Bian, Lu; Lv, Demin; Bi, Shiqi; Dai, Yao; Yang, Kaiyuan; Lu, Hao; Zhou, Huangao; Que, Yunduan; Wang, Dongming; Zhang, Zhijian; Lu, Naiyan.
Afiliação
  • Shi W; School of Medicine, Jiangnan University, Wuxi, Jiangsu, 214122, People's Republic of China.
  • Bian L; School of Medicine, Jiangnan University, Wuxi, Jiangsu, 214122, People's Republic of China.
  • Lv D; School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212001, People's Republic of China.
  • Bi S; School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212001, People's Republic of China.
  • Dai Y; School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212001, People's Republic of China.
  • Yang K; School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212001, People's Republic of China.
  • Lu H; School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212001, People's Republic of China.
  • Zhou H; School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212001, People's Republic of China.
  • Que Y; Nanjing Gaochun People's Hospital, Nanjing, 211300, People's Republic of China.
  • Wang D; Nanjing Gaochun People's Hospital, Nanjing, 211300, People's Republic of China.
  • Zhang Z; School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212001, People's Republic of China.
  • Lu N; State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, People's Republic of China. lunaiyan@jiangnan.edu.cn.
Amino Acids ; 2020 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-33245424
ABSTRACT
As a promising cell therapy, neural crest-derived ectoderm mesenchymal stem cells (EMSCs) secrete high amounts of extracellular matrix (ECM) and neurotrophic factors, promoting neural stem cell (NSC) differentiation into neuronal lineages and aiding tissue regeneration. Additionally, the forced overexpression of secreted proteins can increase the therapeutic efficacy of the secretome. Tissue transglutaminase (TG2) is a ubiquitously expressed member of the transglutaminase family of calcium-dependent crosslinking enzymes, which can stabilize the ECM, inducing smart or living biomaterial to stimulate differentiation and enhance the neurogenesis of NSCs. In this study, we examined the neuronal differentiation of NSCs induced by TG2 gene-modified EMSCs (TG2-EMSCs) in a co-culture model directly. Two weeks after initiating differentiation, levels of the neuronal markers, tubulin beta 3 class III and growth-associated protein 43, were higher in NSCs in the TG2-EMSC co-culture group and those of the astrocytic marker glial fibrillary acidic protein were lower, compared with the control group. These results were confirmed by immunofluorescence, and laminin, fibronectin and sonic hedgehog (Shh) contributed to this effect. The results of western blot analysis and the enzyme-linked immunoassay showed that after TG2-EMSCs were co-cultured for 2 weeks, they expressed much higher levels of Shh than the control group. Moreover, the sustained release of Shh was observed in the TG2-EMSC co-culture group. Overall, our findings indicate that EMSCs can induce the differentiation of NSCs, of which TG2-EMSCs can promote the differentiation of NSCs compared with EMSCs.
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Tipo de estudo: Estudo observacional / Estudo prognóstico Idioma: Inglês Assunto da revista: Bioquímica Ano de publicação: 2020 Tipo de documento: Artigo

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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Tipo de estudo: Estudo observacional / Estudo prognóstico Idioma: Inglês Assunto da revista: Bioquímica Ano de publicação: 2020 Tipo de documento: Artigo