Monocyte activation in persons living with HIV and tuberculosis coinfection.
AIDS
; 35(3): 447-452, 2021 03 01.
Article
em En
| MEDLINE
| ID: mdl-33252496
ABSTRACT
OBJECTIVES:
To characterize monocyte subsets and activation in persons living with HIV (PLWH) with tuberculosis coinfection.DESIGN:
Cross-sectional study within a cohort of PLWH and HIV-uninfected participants at the Joint Clinical Research Centre in Kampala, Uganda.METHODS:
Participants were at least 45 years old with at least one cardiovascular risk factor. PLWH had an HIV viral load 1000âcopies/ml or less on stable antiretroviral therapy prior to cohort entry. QuantiFERON-TB testing was performed to define latent tuberculosis infection (LTBI). Prior active TB was defined by self-report and verified by medical records. Blood was stained with monocyte subset markers (CD14+, CD16), CD62p, CD69, CX3CR1, HLA-DR, and tissue factor, and examined with flow cytometry.RESULTS:
One hundred and twenty-five participants (83 PLWH and 42 without HIV) were included. Median CD4+ count was 582âcells/µl in PLWH. PLWH had a higher frequency of total monocytes (4.3% vs. 3.2%; Pâ<â0.001) and inflammatory monocyte subset (15.5% vs. 11.7%; Pâ=â0.016) compared with HIV-uninfected individuals. No differences in the frequency of monocyte subsets were observed by TB status. Among PLWH, prior active TB was associated with increased frequency of total monocytes compared with LTBI (5.1% vs. 3.7%; Pâ=â0.013). HLA-DR density on monocytes was three-fold higher in PLWH with LTBI or prior TB compared with PLWH without LTBI (Pâ=â0.002). In multivariate analysis, a higher monocyte HLA-DR density remained associated with LTBI or prior TB in PLWH (log-MFI; bâ=â1.17; Pâ<â0.001).CONCLUSION:
Our findings indicate enhanced monocyte activation in PLWH with LTBI or prior active TB, which may contribute to the pathogenesis of noncommunicable diseases in HIV.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tuberculose
/
Infecções por HIV
/
Tuberculose Latente
/
Coinfecção
Tipo de estudo:
Observational_studies
/
Prevalence_studies
/
Risk_factors_studies
Limite:
Humans
/
Middle aged
País/Região como assunto:
Africa
Idioma:
En
Revista:
AIDS
Assunto da revista:
SINDROME DA IMUNODEFICIENCIA ADQUIRIDA (AIDS)
Ano de publicação:
2021
Tipo de documento:
Article