An optimized protocol for combined fluorescent lectin/immunohistochemistry to characterize tissue-specific glycan distribution in human or rodent tissues.
STAR Protoc
; 2(1): 100237, 2021 03 19.
Article
em En
| MEDLINE
| ID: mdl-33364625
ABSTRACT
Lectin histochemical analysis of tissues combined with immunohistochemistry is a valuable tool to characterize and correlate the spatial distribution of glycans with the presence of specific cell types or antigens of interest. The current protocol describes the application of monosaccharide motif specificity of lectin binding to glycan residues to different tissue types. In addition, we describe stereological methods to provide further quantification of the analyzed tissues. For complete details on the use and execution of this protocol, please refer to Mohd Isa et al. (2018), Contessotto et al. (2020), and Samal et al. (2020).
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
/
Imuno-Histoquímica
/
Corantes Fluorescentes
/
Lectinas
Limite:
Animals
/
Humans
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Irlanda