Construction and characterization of an infectious cDNA clone of enterovirus 71: a rapid method for rescuing infectious virus based on stable cells expressing T7 polymerase.

Fu, Meixian; Bai, Jinjin; Gao, Shuang; Chang, Zhangmei; Zhou, Xin; Long, Jian-Er

Fu, Meixian; Bai, Jinjin; Gao, Shuang; Chang, Zhangmei; Zhou, Xin; Long, Jian-Er.

Afiliação

Fu M; Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, 200032, China.
Bai J; Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, 200032, China.
Gao S; Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, 138 Yixueyuan R., Shanghai, 200032, China.
Chang Z; Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, 200032, China.
Zhou X; Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, 200032, China.
Long JE; Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, 200032, China. longjianer@fudan.edu.cn.

Arch Virol ; 166(2): 627-632, 2021 Feb.

Article em En | MEDLINE | ID: mdl-33423081

Assuntos

Clonagem Molecular/métodos; DNA Complementar/genética; RNA Polimerases Dirigidas por DNA/genética; Enterovirus Humano A/genética; Transfecção/métodos; Proteínas Virais/genética; Viroses/genética; Linhagem Celular; Genoma Viral/genética; Humanos; Lentivirus/genética; Reação em Cadeia da Polimerase/métodos; Regiões Promotoras Genéticas/genética; RNA Viral/genética

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Virais / RNA Polimerases Dirigidas por DNA / Viroses / Transfecção / Clonagem Molecular / DNA Complementar / Enterovirus Humano A Limite: Humans Idioma: En Revista: Arch Virol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China País de publicação: Áustria

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