Exposure of Salmonella biofilms to antibiotic concentrations rapidly selects resistance with collateral tradeoffs.
NPJ Biofilms Microbiomes
; 7(1): 3, 2021 01 11.
Article
em En
| MEDLINE
| ID: mdl-33431848
ABSTRACT
Most bacteria in nature exist in biofilms, which are inherently tolerant to antibiotics. There is currently very limited understanding of how biofilms evolve in response to sub-lethal concentrations of antimicrobials. In this study, we use a biofilm evolution model to study the effects of sub-inhibitory concentrations of three antibiotics on Salmonella Typhimurium biofilms. We show that biofilms rapidly evolve resistance to each antibiotic they are exposed to, demonstrating a strong selective pressure on biofilms from low antibiotic concentrations. While all antibiotics tested select for clinical resistance, there is no common mechanism. Adaptation to antimicrobials, however, has a marked cost for other clinically important phenotypes, including biofilm formation and virulence. Cefotaxime selects mutants with the greatest deficit in biofilm formation followed by azithromycin and then ciprofloxacin. Understanding the impacts of exposure of biofilms to antibiotics will help understand evolutionary trajectories and may help guide how best to use antibiotics in a biofilm context. Experimental evolution in combination with whole-genome sequencing is a powerful tool for the prediction of evolution trajectories associated with antibiotic resistance in biofilms.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Salmonella typhimurium
/
Biofilmes
/
Farmacorresistência Bacteriana
/
Antibacterianos
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
NPJ Biofilms Microbiomes
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Reino Unido