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Adventitial remodeling protects against aortic rupture following late smooth muscle-specific disruption of TGFß signaling.
Kawamura, Y; Murtada, S-I; Gao, F; Liu, X; Tellides, G; Humphrey, J D.
Afiliação
  • Kawamura Y; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT, USA; Department of Biomedical Engineering, Yale University, New Haven, CT, USA.
  • Murtada SI; Department of Biomedical Engineering, Yale University, New Haven, CT, USA.
  • Gao F; Department of Surgery, Yale School of Medicine, New Haven, CT, USA.
  • Liu X; Department of Cell Biology, Yale School of Medicine, New Haven, CT, USA.
  • Tellides G; Department of Surgery, Yale School of Medicine, New Haven, CT, USA; Vascular Biology and Therapeutics Program, Yale School of Medicine, New Haven, CT, USA.
  • Humphrey JD; Department of Biomedical Engineering, Yale University, New Haven, CT, USA; Vascular Biology and Therapeutics Program, Yale School of Medicine, New Haven, CT, USA. Electronic address: jay.humphrey@yale.edu.
J Mech Behav Biomed Mater ; 116: 104264, 2021 04.
Article em En | MEDLINE | ID: mdl-33508556
Altered signaling through transforming growth factor-beta (TGFß) increases the risk of aortic dissection in patients, which has been confirmed in mouse models. It is well known that altered TGFß signaling affects matrix turnover, but there has not been a careful examination of associated changes in structure-function relations. In this paper, we present new findings on the rupture potential of the aortic wall following late postnatal smooth muscle cell (SMC)-specific disruption of type I and II TGFß receptors in a mouse model with demonstrated dissection susceptibility. Using a combination of custom computer-controlled biaxial tests and quantitative histology and immunohistochemistry, we found that loss of TGFß signaling in SMCs compromises medial properties but induces compensatory changes in the adventitia that preserve wall strength above that which is needed to resist in vivo values of wall stress. These findings emphasize the different structural defects that lead to aortic dissection and rupture - compromised medial integrity and insufficient adventitial strength, respectively. Relative differences in these two defects, in an individual subject at a particular time, likely reflects the considerable phenotypic diversity that is common in clinical presentations of thoracic aortic dissection and rupture. There is, therefore, a need to move beyond examinations of bulk biological assays and wall properties to cell- and layer-specific studies that delineate pathologic and compensatory changes in wall biology and composition, and thus the structural integrity of the aortic wall that can dictate differences between life and death.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ruptura Aórtica Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: J Mech Behav Biomed Mater Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ruptura Aórtica Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: J Mech Behav Biomed Mater Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Holanda