Multiple cryopreservation-warming cycles, coupled with blastocyst biopsy, negatively affect IVF outcomes.
Reprod Biomed Online
; 42(3): 572-578, 2021 Mar.
Article
em En
| MEDLINE
| ID: mdl-33516664
ABSTRACT
RESEARCH QUESTION Do multiple cryopreservation-warming cycles, coupled with blastocyst biopsy, negatively affect IVF outcomes? DESIGN:
Patients undergoing IVF with homologous single embryo transfer, and who underwent trophectoderm biopsy for preimplantation genetic testing for aneuploidy (PGT-A) between 2013 and 2017, were divided into three groups based on degree of embryonic micromanipulation once-biopsied, once-cryopreserved (group BC, nâ¯=â¯2603), once-biopsied, twice-cryopreserved (group CBC, nâ¯=â¯95) and twice-biopsied, twice-cryopreserved (group BCBC, nâ¯=â¯15). The primary outcome was live birth; secondary outcomes included positive serum pregnancy test, clinical pregnancy and miscarriage.RESULTS:
Group CBC had a significantly lower chance of live birth (adjusted RR 0.57, 95% CI 0.41 to 0.79) and clinical pregnancy (adjusted RR 0.67, 95% CI 0.53 to 0.85) compared with group BC. Miscarriage rates were similar between groups BC and CBC (adjusted RR 1.3, 95% CI 0.64 to 2.7).CONCLUSIONS:
Multiple cryopreservation-warming cycles, coupled with blastocyst biopsy, negatively affect IVF outcomes. Although PGT-A is thought to improve reproductive outcomes on a per transfer basis, caution must be exercised in counselling patients on the possibility of diminishing returns owing to further embryonic micromanipulation after an embryo has been cryopreserved.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Estresse Fisiológico
/
Blastocisto
/
Fertilização in vitro
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Criopreservação
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Diagnóstico Pré-Implantação
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
Limite:
Adult
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Female
/
Humans
/
Pregnancy
País/Região como assunto:
America do norte
Idioma:
En
Revista:
Reprod Biomed Online
Assunto da revista:
MEDICINA REPRODUTIVA
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Estados Unidos