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Immunoassays for the rapid detection of pantothenic acid in pharmaceutical and food products.
Zeng, Lu; Guo, Lingling; Wang, Zhongxing; Xu, Xinxin; Song, Shanshan; Xu, Liguang; Kuang, Hua; Li, Aike; Xu, Chuanlai.
Afiliação
  • Zeng L; State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, PR China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, PR China.
  • Guo L; State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, PR China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, PR China.
  • Wang Z; State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, PR China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, PR China.
  • Xu X; State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, PR China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, PR China.
  • Song S; State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, PR China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, PR China.
  • Xu L; State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, PR China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, PR China.
  • Kuang H; State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, PR China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, PR China. Electronic address: kuangh@jiangnan.edu.cn.
  • Li A; Academy of National Food and Strategic Reserves Administration, No.11 Baiwanzhuang Street, Beijing 100037, PR China. Electronic address: lak@Chinagrain.org.
  • Xu C; State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, PR China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, PR China.
Food Chem ; 348: 129114, 2021 Jun 30.
Article em En | MEDLINE | ID: mdl-33516998
ABSTRACT
Pharmaceutical and food products are fortified with pantothenic acid (PA) to address potential deficiency. Therefore, its fast, reliable, and accurate detection is of great importance to the quality control. Here, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a gold nanoparticle-based lateral flow immunoassay (LFIA) were established for the determination of PA based on an anti-PA monoclonal antibody (mAb). The ic-ELISA displayed a limit of detection (LOD) of 32.22 ng/mL, and the linear range was 64.44-628.84 ng/mL. Average recoveries of PA in fortified samples were 88.60-110.11% when using the ic-ELISA and a good correlation between the ic-ELISA and LC-MS/MS was obtained when analyzing samples. Furthermore, the developed LFIA strip showed a calculated LOD of 71.99, 115.80, and 240.12 ng/mL in B-complex Vitamin tablets, energy drink and infant milk powder samples, respectively. All the results demonstrated that both of these immunoassays are suitable for determining PA in pharmaceutical and food products.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ácido Pantotênico / Imunoensaio / Preparações Farmacêuticas Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Food Chem Ano de publicação: 2021 Tipo de documento: Article País de publicação: ENGLAND / ESCOCIA / GB / GREAT BRITAIN / INGLATERRA / REINO UNIDO / SCOTLAND / UK / UNITED KINGDOM
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ácido Pantotênico / Imunoensaio / Preparações Farmacêuticas Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Food Chem Ano de publicação: 2021 Tipo de documento: Article País de publicação: ENGLAND / ESCOCIA / GB / GREAT BRITAIN / INGLATERRA / REINO UNIDO / SCOTLAND / UK / UNITED KINGDOM