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Optical Monitoring of the Production Quality of Si-Nanoribbon Chips Intended for the Detection of ASD-Associated Oligonucleotides.
Malsagova, Kristina A; Pleshakova, Tatyana O; Popov, Vladimir P; Kupriyanov, Igor N; Galiullin, Rafael A; Kozlov, Andrey F; Shumov, Ivan D; Kaysheva, Anna L; Tikhonenko, Fedor V; Archakov, Alexander I; Ivanov, Yuri D.
Afiliação
  • Malsagova KA; Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia.
  • Pleshakova TO; Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia.
  • Popov VP; Rzhanov Institute of Semiconductor Physics, Siberian Branch of Russian Academy of Sciences, Laboratory of Silicon Material Science, 630090 Novosibirsk, Russia.
  • Kupriyanov IN; Sobolev Institute of Geology and Mineralogy, Siberian Branch of Russian Academy of Sciences, Laboratory of Experimental Mineralogy and Crystallogenesis, 630090 Novosibirsk, Russia.
  • Galiullin RA; Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia.
  • Kozlov AF; Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia.
  • Shumov ID; Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia.
  • Kaysheva AL; Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia.
  • Tikhonenko FV; Rzhanov Institute of Semiconductor Physics, Siberian Branch of Russian Academy of Sciences, Laboratory of Silicon Material Science, 630090 Novosibirsk, Russia.
  • Archakov AI; Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia.
  • Ivanov YD; Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia.
Micromachines (Basel) ; 12(2)2021 Feb 03.
Article em En | MEDLINE | ID: mdl-33546438
Gas-phase etching and optical lithography were employed for the fabrication of a silicon nanoribbon chip (Si-NR chip). The quality of the so-fabricated silicon nanoribbons (Si-NRs) was monitored by optical Raman scattering spectroscopy. It was demonstrated that the structures of the Si-NRs were virtually defect-free, meaning they could be used for highly sensitive detection of biological macromolecules. The Si-NR chips were then used for the highly sensitive nanoelectronics detection of DNA oligonucleotides (oDNAs), which represent synthetic analogs of 106a-5p microRNA (miR-106a-5p), associated with the development of autism spectrum disorders in children. The specificity of the analysis was attained by the sensitization of the Si-NR chip sur-face by covalent immobilization of oDNA probes, whose nucleotide sequence was complementary to the known sequence of miR-106a-5p. The use of the Si-NR chip was demonstrated to al-low for the rapid label-free real-time detection of oDNA at ultra-low (~10-17 M) concentrations.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Risk_factors_studies Idioma: En Revista: Micromachines (Basel) Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Federação Russa País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Risk_factors_studies Idioma: En Revista: Micromachines (Basel) Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Federação Russa País de publicação: Suíça