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Concordance with established tests and reproducibility of results obtained with a new single antigen chip array for HLA antibody detection (HISTO SPOT® HLA AB).
Launhardt, Kristin; Lefevre, Virginie; Souplet, Vianney; Prantl, Livia; Marget, Matthias; Hovoricova, Blanka; Wenda, Sabine; Olivier, Christophe.
Afiliação
  • Launhardt K; BAG Diagnostics GmbH, Amtsgerichtstr 1-5, 35423 Lich, Germany. Electronic address: k.launhardt@bag-diagnostics.com.
  • Lefevre V; Innobiochips, 70, rue du Docteur Yersin, 59 120 Loos, France.
  • Souplet V; Innobiochips, 70, rue du Docteur Yersin, 59 120 Loos, France.
  • Prantl L; Central Institute for Blood Transfusion and Immunology, University Hospital, Anichstr. 35, 6020 Innsbruck, Austria.
  • Marget M; Institute for Transfusion Medicine, University Hospital Hamburg Eppendorf, Martinistr. 52, 20246 Hamburg, Germany.
  • Hovoricova B; University Hospital F. D. Roosevelta Banska Bystrica, Department of Laboratory Hematology - HLA Laboratory, Namestie L. Svobodu 1, 975 17, Banska Bystrica, Slovakia.
  • Wenda S; Medical University Vienna, University Hospital for Blood Group Serology and Transfusion Medicine, Währinger Gürtel 18-20 / Ebene 4i, 1090 Vienna, Austria.
  • Olivier C; Innobiochips, 70, rue du Docteur Yersin, 59 120 Loos, France.
J Immunol Methods ; 491: 112971, 2021 04.
Article em En | MEDLINE | ID: mdl-33549571
ABSTRACT
The purpose of this study was to analyze performance of a new single antigen chip array system (HISTO SPOT® HLA AB) developed for HLA antibody detection and compare with results obtained using single antigen Luminex-based systems and serum samples from the Eurotransplant external proficiency testing scheme. Results were analysed from 11 independent Eurotransplant laboratories using HISTO SPOT® HLA AB utilising the Eurotransplant external proficiency testing (EPT) sera and these were compared to published results from 67 labs using the Luminex-based technologies. In addition, QC results from different batches of the test were analysed. Generally, concordance of results with the results from the Luminex technique was good. With the Luminex tests more consensus results and more questionable results were found than with the HISTO SPOT® HLA AB test. Within the HISTO SPOT® HLA AB testing group we found a discrepancy rate from the consensus of 2.9% for the EPT sera which is far below the 25% allowed to pass the quality test and only slightly higher than for the Luminex single antigen tests with 1.2%. The average global coefficient of variation (CV) of the mean signal (raw data) for the HISTO SPOT® HLA AB test was 13% which is lower than the values reported for Luminex tests in the literature. The average global CV for the signal/background ratio was higher with 28%. In the present study, the mean signal is the best parameter to compare results between labs and the new HISTO SPOT® HLA AB test is at least as good in terms of signal reproducibility as the Luminex tests. In conclusion, the HISTO SPOT® HLA AB test is a good alternative to be used in addition or instead of the Luminex tests in clinical labs.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Imunoensaio / Antígenos HLA / Isoanticorpos Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Revista: J Immunol Methods Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Imunoensaio / Antígenos HLA / Isoanticorpos Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Revista: J Immunol Methods Ano de publicação: 2021 Tipo de documento: Article