Pyruvate kinase-deficiency anemia: membrane approach.
Biochem Med Metab Biol
; 39(1): 55-63, 1988 Feb.
Article
em En
| MEDLINE
| ID: mdl-3355716
ABSTRACT
Low ionic strength extraction (37 degrees C, 30 min) of ghosts from PK-deficient erythrocytes provided crude spectrin extract. No significant differences in the extract composition compared to normal donors were observed. The reticulocyte-dependent spectrin extractability was found among the subjects with PK-deficiency anemia. Likewise ATP-depletion affects spectrin extractability and also leads to the adsorption of cytoplasmic protein MW 50,000 to the reticulocyte membrane. The measurement of membrane fluidity using the fluorescence probe DPH did not reveal significant alterations in the moiety of integral membrane constituents.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Piruvato Quinase
/
Eritrócitos
/
Anemia Hemolítica
Limite:
Humans
Idioma:
En
Revista:
Biochem Med Metab Biol
Assunto da revista:
BIOQUIMICA
Ano de publicação:
1988
Tipo de documento:
Article