Tanshinone I and Tanshinone IIA/B attenuate LPS-induced mastitis via regulating the NF-κB.
Biomed Pharmacother
; 137: 111353, 2021 May.
Article
em En
| MEDLINE
| ID: mdl-33578236
ABSTRACT
BACKGROUND:
Mastitis is a common disease occurs in breast-feeding mothers, but published data are poor. This study aimed to study the effects of Tanshinones on treating mastitis.METHODS:
Clinical trials performed in 58 breast-feeding mothers were carried out. B-ultrasound and blood test were used to measure the size of breast mass and the change of blood cell counts. BALB/c mice were injected with LPS and then treated by Tanshinone I or Tanshinone IIA/B. Myeloperoxidase (MPO) activity and the release of inflammatory cytokines were tested by MPO kit, RT-qPCR and ELISA. Mouse mammary epithelial cells (mMECs) were isolated and the effects of Tanshinones were measured by conducting CCK-8 assay, flow cytometry, RT-qPCR and ELISA.RESULTS:
Patients treated by Cefprozil combined with Tanshinone got better outcomes than patients treated by Cefprozil alone. In animal trials, Tanshinone I and Tanshinone IIA/B significantly reduced MPO activity, and the levels of TNF-α, IL-1ß and IL-6 in serum and mammary gland tissues. In mMECs, Tanshinone I and Tanshinone IIA/B attenuated LPS-induced viability loss and apoptosis. And they effectively inhibited the release of TNF-α, IL-1ß and IL-6. Also, Tanshinone I and Tanshinone IIA/B significantly attenuated LPS-evoked NF-κB activation.CONCLUSION:
Tanshinone I and Tanshinone IIA/B have potentials in treating mastitis. The beneficial effects might be through regulating NF-κB activation.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Abietanos
/
Subunidade p50 de NF-kappa B
/
Mastite
/
Anti-Infecciosos
Tipo de estudo:
Clinical_trials
/
Prognostic_studies
Idioma:
En
Revista:
Biomed Pharmacother
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China