[Development and experience in the use of an immunoenzyme test system for determining specific antihemolysins in patients with measles]. / Razrabotka i opyt primeneniia immunofermentnoi test-sistemy dlia opredeleniia spetsificheskikh antigemolizinov u bol'nykh kor'iu.
Zh Mikrobiol Epidemiol Immunobiol
; (2): 95-8, 1988 Feb.
Article
em Ru
| MEDLINE
| ID: mdl-3364085
ABSTRACT
To determine the physico-chemical nature of specific antimeasles antihemolysins, an enzyme immunoassay (EIA) system with the use of stable measles virus hemolysing antigen has been developed. The expedient method has been worked out the antigen diluted 120 with the initial hemolytic activity in the direct hemolysis inhibition test equal to 164 and, for its fixation, 0.1 M carbonate-bicarbonate buffer solution, pH 9.6, are used; the fixation of the antigen is carried out at 4 degrees C for 16-20 hours. The final dilution of the serum, whose coloration significantly differs from that of the control, is considered to be the titer of antimeasles antihemolysins. Specific antihemolysins belonging to three classes of immunoglobulins, A, M and G, are synthesized in measles. The detection of IgM-antihemolysins in high titers on the first day of rash opens prospects for using the newly developed EIA system for the rapid diagnosis of measles.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Hemolisinas
/
Sarampo
/
Vírus do Sarampo
/
Anticorpos Antivirais
/
Especificidade de Anticorpos
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Adolescent
/
Child
/
Child, preschool
/
Humans
Idioma:
Ru
Revista:
Zh Mikrobiol Epidemiol Immunobiol
Ano de publicação:
1988
Tipo de documento:
Article