Pseudomonas cyclaminis sp. nov., a pathogen causing bacterial bud blight of cyclamen in Japan.
Int J Syst Evol Microbiol
; 71(3)2019 Jun.
Article
em En
| MEDLINE
| ID: mdl-33650945
ABSTRACT
Five phytopathogenic bacterial strains, MAFF 301449T, MAFF 301450, MAFF 301451, MAFF 301452, and MAFF 301453, which were isolated from bud blight lesions of cyclamen (Cyclamen persicum Mill.) in Miyagi, Japan, were subjected to polyphasic taxonomic characterisation. The cells were Gram-reaction-negative, aerobic, non-spore-forming, motile with one to five polar flagella, and rod-shaped. Analysis of 16S rRNA gene sequences showed that they belong to the genus Pseudomonas, with Pseudomonas extremaustralis 14-3T (99.79â% sequence similarity), Pseudomonas trivialis DSM 14937T (99.79â%), Pseudomonas poae DSM 14936T (99.72â%), and Pseudomonas antarctica CMS 35T (99.72â%) as their relatives. The genomic DNA G+C content was 60.3 mol% and the major fatty acids (>5â% of the total fatty acids) were C16ââ0, summed feature 3 (C16ââ1 ω7c/C16ââ1 ω6c), summed feature 8 (C18ââ1 ω7c/C18ââ1 ω6c), and C17ââ0 cyclo. Phylogenetic analysis using the rpoD gene sequences and phylogenomic analyses based on the whole genome sequences demonstrated that the strains are members of the Pseudomonas fluorescens subgroup, but form a monophyletic and robust clade separated from their relatives. Average nucleotide identity and digital DNA-DNA hybridisation analyses with the closely related Pseudomonas species corroborated their novel species status. The strains were differentiated from their relatives by phenotypic characteristics, pathogenicity towards cyclamen, cellular fatty acid composition, and whole-cell MALDI-TOF mass spectrometry profiles. Based on the phenotypic, chemotaxonomic, and genotypic data obtained, we conclude that the strains represent a novel Pseudomonas species, for which we propose the name Pseudomonas cyclaminis sp. nov.; the type strain is MAFF 301449T (=ICMP 23720T).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Japão