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Visualizing Cholesterol in the Brain by On-Tissue Derivatization and Quantitative Mass Spectrometry Imaging.
Angelini, Roberto; Yutuc, Eylan; Wyatt, Mark F; Newton, Jillian; Yusuf, Fowzi A; Griffiths, Lauren; Cooze, Benjamin J; El Assad, Dana; Frache, Gilles; Rao, Wei; Allen, Luke B; Korade, Zeljka; Nguyen, Thu T A; Rathnayake, Rathnayake A C; Cologna, Stephanie M; Howell, Owain W; Clench, Malcolm R; Wang, Yuqin; Griffiths, William J.
Afiliação
  • Angelini R; Medical School, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, U.K.
  • Yutuc E; Medical School, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, U.K.
  • Wyatt MF; Medical School, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, U.K.
  • Newton J; Centre for Mass Spectrometry Imaging, Biomolecular Research Centre, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, U.K.
  • Yusuf FA; Medical School, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, U.K.
  • Griffiths L; Medical School, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, U.K.
  • Cooze BJ; Medical School, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, U.K.
  • El Assad D; Materials Research and Technology, Luxembourg Institute of Science and Technology, Belvaux L-4422, Luxembourg.
  • Frache G; Materials Research and Technology, Luxembourg Institute of Science and Technology, Belvaux L-4422, Luxembourg.
  • Rao W; European Application Laboratory, Waters Corporation, Stamford Avenue, Altrincham Road, Wilmslow SK9 4AX, U.K.
  • Allen LB; Departments of Pediatrics and Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska 68198, United States.
  • Korade Z; Departments of Pediatrics and Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska 68198, United States.
  • Nguyen TTA; Department of Chemistry and Laboratory of Integrated Neuroscience, University of Illinois at Chicago, Chicago, Illinois 60607, United States.
  • Rathnayake RAC; Department of Chemistry and Laboratory of Integrated Neuroscience, University of Illinois at Chicago, Chicago, Illinois 60607, United States.
  • Cologna SM; Department of Chemistry and Laboratory of Integrated Neuroscience, University of Illinois at Chicago, Chicago, Illinois 60607, United States.
  • Howell OW; Medical School, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, U.K.
  • Clench MR; Centre for Mass Spectrometry Imaging, Biomolecular Research Centre, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, U.K.
  • Wang Y; Medical School, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, U.K.
  • Griffiths WJ; Medical School, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, U.K.
Anal Chem ; 93(11): 4932-4943, 2021 03 23.
Article em En | MEDLINE | ID: mdl-33687199
ABSTRACT
Despite being a critical molecule in the brain, mass spectrometry imaging (MSI) of cholesterol has been under-reported compared to other lipids due to the difficulty in ionizing the sterol molecule. In the present work, we have employed an on-tissue enzyme-assisted derivatization strategy to improve detection of cholesterol in brain tissue sections. We report distribution and levels of cholesterol across specific structures of the mouse brain, in a model of Niemann-Pick type C1 disease, and during brain development. MSI revealed that in the adult mouse, cholesterol is the highest in the pons and medulla and how its distribution changes during development. Cholesterol was significantly reduced in the corpus callosum and other brain regions in the Npc1 null mouse, confirming hypomyelination at the molecular level. Our study demonstrates the potential of MSI to the study of sterols in neuroscience.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Colesterol / Doença de Niemann-Pick Tipo C Limite: Animals Idioma: En Revista: Anal Chem Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Colesterol / Doença de Niemann-Pick Tipo C Limite: Animals Idioma: En Revista: Anal Chem Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Reino Unido