The thermal stability of oligonucleotide duplexes is sequence independent in tetraalkylammonium salt solutions: application to identifying recombinant DNA clones.
Nucleic Acids Res
; 16(10): 4637-50, 1988 May 25.
Article
em En
| MEDLINE
| ID: mdl-3380691
ABSTRACT
In solutions of tetraalkylammonium salts the melting temperature of oligonucleotide duplexes is independent of nucleotide sequence and thus GC content. Data quantitating the destabilizing effects of various mismatches in these solvents are also presented. The results are in accord with theories on DNA melting and establish conditions under which oligonucleotides can be used as hybridization probes with predictable and controllable specificity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligodesoxirribonucleotídeos
/
DNA Recombinante
Idioma:
En
Revista:
Nucleic Acids Res
Ano de publicação:
1988
Tipo de documento:
Article