Urinary l-erythro-ß-hydroxyasparagine-a novel serine racemase inhibitor and substrate of the Zn2+-dependent d-serine dehydratase.
Biosci Rep
; 41(4)2021 04 30.
Article
em En
| MEDLINE
| ID: mdl-33821987
In the present study, we identified l-erythro-ß-hydroxyasparagine (l-ß-EHAsn) found abundantly in human urine, as a novel substrate of Zn2+-dependent d-serine dehydratase (DSD). l-ß-EHAsn is an atypical amino acid present in large amounts in urine but rarely detected in serum or most organs/tissues examined. Quantitative analyses of urinary l-ß-EHAsn in young healthy volunteers revealed significant correlation between urinary l-ß-EHAsn concentration and creatinine level. Further, for in-depth analyses of l-ß-EHAsn, we developed a simple three-step synthetic method using trans-epoxysuccinic acid as the starting substance. In addition, our research revealed a strong inhibitory effect of l-ß-EHAsn on mammalian serine racemase, responsible for producing d-serine, a co-agonist of the N-methyl-d-aspartate (NMDA) receptor involved in glutamatergic neurotransmission.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Asparagina
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L-Serina Desidratase
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Urina
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Racemases e Epimerases
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Inibidores Enzimáticos
Limite:
Animals
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Humans
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Male
Idioma:
En
Revista:
Biosci Rep
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Japão
País de publicação:
Reino Unido