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ePCL Electrospun Microfibrous Layers for Immune Assays: Sensitive ELISA for the Detection of Serum Antibodies Against HPV16 E7 Oncoprotein.
Falcucci, Susanna; Paolini, Francesca; Mileo, Anna Maria; Franconi, Rosella; Massa, Silvia; Rinaldi, Antonio; Venuti, Aldo.
Afiliação
  • Falcucci S; HPV-Unit UOSD Tumor Immunology and Immunotherapy-IRCCS Regina Elena National Cancer Institute, Via Elio Chianesi 53, 00144 Rome, Italy.
  • Paolini F; HPV-Unit UOSD Tumor Immunology and Immunotherapy-IRCCS Regina Elena National Cancer Institute, Via Elio Chianesi 53, 00144 Rome, Italy.
  • Mileo AM; UOSD Tumor Immunology and Immunotherapy-IRCCS Regina Elena National Cancer Institute, Via Elio Chianesi 53, 00144 Rome, Italy.
  • Franconi R; Department of Sustainability, ENEA (Italian National Agency for New Technologies, Energy and Sustainable Economic Development), Casaccia Research Centre, Via Anguillarese, 301, S. Maria di Galeria, 00123 Rome, Italy.
  • Massa S; Department of Sustainability, ENEA (Italian National Agency for New Technologies, Energy and Sustainable Economic Development), Casaccia Research Centre, Via Anguillarese, 301, S. Maria di Galeria, 00123 Rome, Italy.
  • Rinaldi A; Department of Sustainability, ENEA (Italian National Agency for New Technologies, Energy and Sustainable Economic Development), Casaccia Research Centre, Via Anguillarese, 301, S. Maria di Galeria, 00123 Rome, Italy.
  • Venuti A; HPV-Unit UOSD Tumor Immunology and Immunotherapy-IRCCS Regina Elena National Cancer Institute, Via Elio Chianesi 53, 00144 Rome, Italy.
ACS Omega ; 6(13): 8778-8783, 2021 Apr 06.
Article em En | MEDLINE | ID: mdl-33842749
ABSTRACT
Human papillomavirus (HPV) type 16 is the etiologic agent of more than 50% anal/cervical cancers and about 20% oropharyngeal cancers. HPV16 E6 and E7 oncogenes favor the transformation and are essential for maintaining the transformed status. Serum anti-E6 and anti-E7 antibodies appear to have prognostic significance for HPV-associated cancers. However, most of the previous attempts to establish diagnostic tools based on serum detection of E6 and/or E7 antibodies have been unsuccessful, mainly due to the low accuracy of applied tests. This paper reports on a feasibility study to prove the possibility to easily immobilize HPV16 E7 onto electrospun substrates for application in diagnostic tools. In this study, poly(ε-caprolactone) electrospun scaffolds (called ePCL) are used to provide a microstructured substrate with a high surface-to-volume ratio, capable of binding E7 proteins when used for enzyme-linked immunosorbent assay (ELISA) tests. ePCL functionalized with E7 exhibited superior properties compared to standard polystyrene plates, increasing the detection signal from serum antibodies by 5-6 times. Analysis of the serum samples from mice immunized with HPV16 E7 DNA vaccine showed higher efficiency of this new anti-E7 ePCL-ELISA test vs control in E7-specific antibody detection. In addition, ePCL-E7-ELISA is prepared with a relatively low amount of antigen, decreasing the manufacturing costs.

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies Idioma: En Revista: ACS Omega Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Itália

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies Idioma: En Revista: ACS Omega Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Itália