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A dual-site controlled pH probe revealing the pH of sperm cytoplasm and screening for healthy spermatozoa.
Li, Xiaoliang; Wu, Sixian; Yu, Kangkang; Hou, Jianwen; Jiang, Chuan; Li, Kun; Chu, Zhiwen; Jiang, Xiaohui; Yu, Xiaoqi; Xu, Wenming.
Afiliação
  • Li X; Department of Obstetrics/Gynecology, Joint Laboratory of Reproductive Medicine, SCU-CUHK, Key Laboratory of Obstetric, Gynaecologic and Paediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, 610041, China. xuwenming@scu.edu
  • Wu S; Department of Obstetrics/Gynecology, Joint Laboratory of Reproductive Medicine, SCU-CUHK, Key Laboratory of Obstetric, Gynaecologic and Paediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, 610041, China. xuwenming@scu.edu
  • Yu K; Key Laboratory of Bio-Resources and Eco-Environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu, 610064, China.
  • Hou J; Department of Obstetrics/Gynecology, Joint Laboratory of Reproductive Medicine, SCU-CUHK, Key Laboratory of Obstetric, Gynaecologic and Paediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, 610041, China. xuwenming@scu.edu
  • Jiang C; Department of Obstetrics/Gynecology, Joint Laboratory of Reproductive Medicine, SCU-CUHK, Key Laboratory of Obstetric, Gynaecologic and Paediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, 610041, China. xuwenming@scu.edu
  • Li K; Key Laboratory of Green Chemistry and Technology (Ministry of Education), College of Chemistry, Sichuan University, Chengdu, 610064, China. xqyu@scu.edu.cn.
  • Chu Z; West China School of Pharmacy, Sichuan University, Chengdu, 610064, China.
  • Jiang X; Human Sperm Bank, West China Second University Hospital, Sichuan University, Chengdu, 610041, China.
  • Yu X; Key Laboratory of Green Chemistry and Technology (Ministry of Education), College of Chemistry, Sichuan University, Chengdu, 610064, China. xqyu@scu.edu.cn.
  • Xu W; Department of Obstetrics/Gynecology, Joint Laboratory of Reproductive Medicine, SCU-CUHK, Key Laboratory of Obstetric, Gynaecologic and Paediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, 610041, China. xuwenming@scu.edu
J Mater Chem B ; 9(17): 3662-3665, 2021 05 05.
Article em En | MEDLINE | ID: mdl-33870986
ABSTRACT
A dual-site controlled pH probe, which is composed of gold nanoparticles and modified with rhodamine and fluorescein derivatives, was applied to sensitively monitor intracellular pH changes in sperm. The pH probe revealed the intracellular pH of sperm under different conditions and demonstrated the lower pH in asthenozoospermia patients as compared to healthy individuals. Importantly, the pH probe can help screen for healthy sperm.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espermatozoides / Citoplasma / Astenozoospermia Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Humans / Male Idioma: En Revista: J Mater Chem B Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China País de publicação: ENGLAND / ESCOCIA / GB / GREAT BRITAIN / INGLATERRA / REINO UNIDO / SCOTLAND / UK / UNITED KINGDOM

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espermatozoides / Citoplasma / Astenozoospermia Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Humans / Male Idioma: En Revista: J Mater Chem B Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China País de publicação: ENGLAND / ESCOCIA / GB / GREAT BRITAIN / INGLATERRA / REINO UNIDO / SCOTLAND / UK / UNITED KINGDOM