The motility of human milk macrophages in collagen gels.
Pediatr Res
; 23(5): 449-52, 1988 May.
Article
em En
| MEDLINE
| ID: mdl-3387165
ABSTRACT
The hypothesis that the diminished motility of human milk leukocytes is due to a decrease in adherence was tested by using a collagen gel system in which leukocyte movement is less dependent on adherence. Unfractionated human milk leukocytes (HML) or fractionated peripheral blood leukocytes were placed on collagen gels in microwells and the leading edge of migration was determined by inverted phase microscopy. The mean rates of invasion of HML, blood neutrophils, and mononuclear blood leukocytes were 14, 240, and less than 1 mu/h, respectively (p less than 0.01). We then examined the identity of motile HML by immunoperoxidase techniques using antibodies to selected cell markers. Motile HML were positive for a specific macrophage marker (cathepsin B) and a neutrophil and monocyte marker (Mac-1) but were negative for specific neutrophil (cathepsin G) or lymphocyte markers (CD3 and CD5). The directed motility of these cells was not enhanced by exposure to the chemoattractant, N-formyl-L-methionyl-L-phenylalanine, but was inhibited by a T cell lectin, phytohemagglutinin. The movement was actin dependent but was not dependent on calcium or Mac-1 surface glycoproteins. Thus, the diminished motility of milk neutrophils does not appear to be due to decreased adherence per se, and those HML that are motile are macrophages. This suggests a dichotomy for the function of HML. Neutrophils may be relegated to the lumen of th alimentary tract, whereas macrophages may penetrate into mucosal sites for host defense.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Inibição de Migração Celular
/
Colágeno
/
Leucócitos
/
Leite Humano
Limite:
Humans
Idioma:
En
Revista:
Pediatr Res
Ano de publicação:
1988
Tipo de documento:
Article