Cytokinins in the xylem sap of the dioecious fynbos shrub, Leucadendron rubrum Burm. f.: seasonal fluctuations and their possible interaction with morphological characteristics as expressed in the two sexes.

Brandies of male and female plants of the dioecious Leucadendron rubrum Burm. f. were collected on the site of the Weikamp weather-station in the Jonkershoek State Forest, Stellenbosch, from July 1990 to September 1991. Xylem sap was extracted from the branches by vacuum extraction. HPLC was employed to purify the xylem sap and to separate the cytokinins prior to quantitation by ELISA. The cytokinin levels in the purified plant samples were determined using rabbit antiserum-based ELISAs for the determination of the levels of the cytokinins DHZR and IPA, and a trans-ZR specific monoclonal antibody-based ELISA for the determination of the levels of the cytokinin ZR. It was observed that the levels of the cytokinins DHZR and ZR did not differ significantly between male and female plants but did correlate with the seasonal growth patterns. The levels of the cytokinin IPA, in male and female plants of L. rubrum, not only correlated specifically with the different seasonal growth patterns observed but differed significantly between male and female plants. Peaks in the levels of all three cytokinins. i.e. ZR, DHZR and IPA, occurred during the study period. These peaks corresponded with the phenological development of L. rubrum, specifically with vegetative bud break and inflorescence differentiation and appeared to be synchronized with the growth and development. An attempt was also made to correlate cytokinin levels with the extreme differences in morphological characters exhibited by male and female plants, and possibly to explain to what extent these phytohormones influence sex expression in L. rubrum.