Development and use of a reverse transcription insulated isothermal PCR assay for detection and characterization of bovine torovirus in yaks.
Arch Virol
; 166(7): 2017-2025, 2021 Jul.
Article
em En
| MEDLINE
| ID: mdl-33881617
ABSTRACT
Bovine torovirus (BToV) is an important diarrhea-causing pathogen affecting bovines. To facilitate BToV detection, a reverse transcription insulated isothermal PCR (RT-iiPCR) assay was developed that targets the BToV M gene with high specificity and reproducibility. The assay has a limit of detection of 23 copies/µL. Out of 69 diarrheic fecal samples from yaks collected on six farms in Tibet and Sichuan provinces in China, 11.59% (8/69) tested positive for BToV using this assay. The full-length spike (S) and hemagglutinin-esterase (HE) genes of three positive samples were subsequently sequenced. Notably, an identical recombination event was identified in the S1 subunit of the S protein of three isolates. All of the HE genes were found to belong to genotype III and shared the same unique aa variation (P44S) in the esterase domain. This study is the first confirmation of BToV in yaks and the first report of an S gene recombination event in BToV. Our findings will enhance the current understanding of the molecular characteristics and genetic evolution of BToV.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças dos Bovinos
/
Infecções por Torovirus
/
Torovirus
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Transcrição Reversa
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Revista:
Arch Virol
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China