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Comparative Cytogenetics in Four Leptodactylus Species (Amphibia, Anura, Leptodactylidae): Evidence of Inner Chromosomal Diversification in Highly Conserved Karyotypes.
da Silva, David S; da Silva Filho, Heriberto F; Cioffi, Marcelo B; de Oliveira, Edivaldo H C; Gomes, Anderson J B.
Afiliação
  • da Silva DS; Laboratório de Biologia Molecular, Evolução e Microbiologia, Instituto Federal do Pará, Abaetetuba, Brazil.
  • da Silva Filho HF; Laboratório de Biologia Celular e Helmintologia, Universidade Federal do Pará, Belém, Brazil.
  • Cioffi MB; Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, Brazil.
  • de Oliveira EHC; Laboratório de Cultura de Tecidos e Citogenética, SAMAM, Instituto Evandro Chagas, Ananindeua, Brazil.
  • Gomes AJB; Faculdade de Ciências Naturais, ICEN, Universidade Federal do Pará, Belém, Brazil.
Cytogenet Genome Res ; 161(1-2): 52-62, 2021.
Article em En | MEDLINE | ID: mdl-33887732
With 82 species currently described, the genus Leptodactylus is the most diverse and representative one in the family Leptodactylidae. Concerning chromosomal organization, this genus represents an interesting and underexplored group since data from molecular cytogenetics are incipient, and little is known about the organization and distribution of repetitive DNA elements in the karyotypes. In this sense, this study aimed at providing a comparative analysis in 4 Leptodactylus species (L. macrosternum, L. pentadactylus, L. fuscus, and Leptodactylus cf. podicipinus), combining conventional cytogenetics (Giemsa staining, C-banding, and AgNOR staining) and mapping of molecular markers (18S rDNA, telomeric and microsatellite probes), to investigate mechanisms underlying their karyotype differentiation process. The results showed that all species had karyotypes with 2n = 22 and FN = 44, except for Leptodactylus cf. podicipinus which presented FN = 36. The 18S rDNA was observed in pair 8 of all analyzed species (corresponding to pair 4 in L. pentadactylus), coinciding with the secondary constrictions and AgNOR staining. FISH with microsatellite DNA probes demonstrated species-specific patterns, as well as an association of these repetitive sequences with constitutive heterochromatin blocks and ribosomal DNA clusters, revealing the dynamics of microsatellites in the genome of the analyzed species. In summary, our data demonstrate an ongoing process of genomic divergence inside species with almost similar karyotype, driven most likely by a series of pericentric inversions, followed by differential accumulation of repetitive sequences.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Anuros / DNA Ribossômico / Cromossomos / Repetições de Microssatélites / Cariotipagem Limite: Animals Idioma: En Revista: Cytogenet Genome Res Assunto da revista: GENETICA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Anuros / DNA Ribossômico / Cromossomos / Repetições de Microssatélites / Cariotipagem Limite: Animals Idioma: En Revista: Cytogenet Genome Res Assunto da revista: GENETICA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça