Quantification of Enterohemorrhagic Escherichia coli via Optical Nanoantenna and Temperature-responsive Artificial Antibodies.
Anal Sci
; 37(11): 1597-1601, 2021 Nov 10.
Article
em En
| MEDLINE
| ID: mdl-33994418
ABSTRACT
Enterohemorrhagic Escherichia coli are a dangerous bacterium known to be harmful to the human body, with some infections even resulting in death. Given this danger, food factories are required to perform a quick bacterial test to confirm the absence of this pathogen prior to shipping. We have developed a novel molecular imprinting polymer (MIP) particle that has encapsulated gold nanoparticles (AuNPs) and which can function as both a receptor and an optical signal transmitter in biological systems. This MIP particle is artificially synthesized and can be engineered to specifically recognize and capture antigens on the bacterial cell membrane. In addition, MIP particles containing AuNPs generate strong scattered light signals, and binding of the MIP particles improves the optical intensity of the target bacterial cells. This enables clear visualization under a dark-field microscope and quantification of the target bacteria using the scattering light intensity. Here we describe the successful quantification of Escherichia coli O157 cells in real meat samples using this technology in conjunction with a simple labelling step.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Escherichia coli O157
/
Nanopartículas Metálicas
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Escherichia coli Êntero-Hemorrágica
Limite:
Humans
Idioma:
En
Revista:
Anal Sci
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Japão
País de publicação:
CH
/
SUIZA
/
SUÍÇA
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SWITZERLAND